human anti-cd98 igg  (Absolute Biotech Inc)

Journal: STAR Protocols

Article Title: A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples

doi: 10.1016/j.xpro.2021.100924

Figure Lengend Snippet: Sample plating layout To aid in the differentiation of control reactions from sample reactions, designate a row within a plate to controls (top row), followed by samples. Cells within the control wells will not be used to screen biological samples. The IgG Positive control will receive both the anti-CD98 antibody, as well as the staining cocktail. Single color controls, in concert with the unstained control will provide preliminary guidance as to where to draw positive-event gates. The placements of these gates are later refined by the boundaries set by the fluorescence-minus-one controls. We separated healthy human control samples from biological human samples of interest. These healthy human controls can further refine the lower limits of positive-event gates, as it is hypothesized that healthy human controls lack or have minimal circulating autoantibodies.

Article Snippet: Human anti-CD98 IgG; Absolute Antibody Clone CP1-5, Catalog number: Ab00361–10.0).

Techniques: Positive Control, Staining, Fluorescence

Journal: STAR Protocols

Article Title: A flow-cytometry-based protocol using diverse cell types for detecting autoantibodies from human plasma and serum samples

doi: 10.1016/j.xpro.2021.100924

Figure Lengend Snippet:

Article Snippet: Human anti-CD98 IgG; Absolute Antibody Clone CP1-5, Catalog number: Ab00361–10.0).

Techniques: Recombinant, Electron Microscopy, Flow Cytometry